Cas12

CRISPR Beyond the Cut: How Diagnostics and Gene Regulation Use Different Molecular Machinery

CRISPR technology extends far beyond gene editing, utilizing modified enzymes like dCas9 for precise gene regulation and epigenome modification without cutting DNA. Other CRISPR systems, such as Cas12 and Cas13, are employed in diagnostics by leveraging collateral cleavage to detect nucleic acids rapidly and sensitively.